High-resolution structure of cas13b and biochemical characterization of RNA targeting and cleavage

(Cell Reports 26, 3741–3751.e1–e5; March 2019) In our paper, we reported the structure and biochemical characterization of RNA-guided RNase Cas13b from Prevotella buccae (PbuCas13b). This work included an assessment turnover rate this enzyme. Recently, it was brought to attention by Juan Santiago Ashwin Ramachandran that inconsistent with data (Figure 5D). Upon re-evaluation raw data, found error in units. The corrected plot now appears online is shown below, 0.955 s-1. does not affect conclusions paper regard either or mechanism action. We apologize for any confusion may have caused. High-Resolution Structure Biochemical Characterization RNA Targeting CleavageSlaymaker et al.Cell ReportsMarch 2019In BriefSlaymaker al. present a high-resolution combination studies showing highly dynamic, multi-turnover enzyme distinct other Cas13 family members. Additionally, authors demonstrate can be rationally engineered change cleavage specificity create minimal variant suitable delivery viral vectors. Full-Text PDF Open Access